UNS Conference Portal, The 1st International Conference on Science, Mathematics, Environment and Education 2017

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IFN- +874A + IL-10 -1082G Predominant in Javanese HIV Patients with anti-Toxoplasma IgM Negative - IgG Positive
Yulia Sari, Afiono Agung Prasetyo, Hartono Hartono, Zainal Arifin Adnan

Last modified: 2017-07-16

Abstract


Interferon gamma (IFN-γ) and Interleukin 10 (IL-10) are involved in the immunopathogenesis of Toxoplasma gondii infection. The expression of both cytokines were associated with polymorphisms of the respected genes, e.g. IFN-γ +874A/T and IL-10 -1082A/G, respectively. Previously, our research group reported the respected polymorphism status was associated with anti-Toxoplasma seropositivity. However, the association of IFN-γ +874A/T and IL-10 -1082A/G polymorphism status in allele’s combination manner with Toxoplasma gondii infection in HIV patients still unclear. Blood samples obtained from Javanese HIV patients were examined for anti-Toxoplasma IgM and IgG antibodies, respectively, by enzyme linked immunosorbent assays. The genomic DNA were extracted from all blood samples, and subjected for the IFN-γ +874A/T and IL-10 -1082A/G polymorphisms detection by the polymerase chain reaction-sequence-specific primers (PCR-SSPs) technique. PCR products were analyzed in 3% agarose. Anti-Toxoplasma IgM and IgG positivity rates were 10.7% and 30.7%, respectively. In anti-Toxoplasma IgM negative - IgG positive samples, IFN-γ +874A + IL-10 -1082G alleles combination was predominant (90.9%), followed by IFN-γ +874A + IL-10 -1082A (72.7%), IFN-γ +874T + IL-10 -1082G (40.9%), and IFN-γ +874T + IL-10 -1082A (34.1%), respectively. The present results indicated dynamic roles of the IFN-γ +874A/T and IL-10 -1082A/G alleles combination in Toxoplasma gondii susceptibility.